DNA BASED TECHNOLOGY

The Molecular Biology Core (MBC) offers state-of-the-art technology for the research of infectious diseases such as HIV, HCV, and HBV. The Core’s primary function is to provide services to the ARP’s molecular analysis researchers. In addition, the Core trains research technologists and graduate students as well as maintaining the facility for the use of program investigators. The Core is located within a restricted-access area and practices all safety measures required to work with highly biohazardous materials. The well-trained personnel perform techniques such as PCR, RT-PCR, molecular cloning, automated DNA sequencing, and real-time quantitative RT-PCR.

Some of the facilities and equipment available at the Molecular Biology Core are:

DNA Sequencing:

Three automated UNIX-based sequencing units from Visible Genetics.

MicroGene Clipper System

PCR/RT-PCR System:

Two thermocycler units with temperature gradient capability for faster optimization of protocols. Two single block thermocycler units (used for most PCR or RT-PCR applications). Two BioRad iCycler with a real-time quantitative PCR cycler unit. Two vertical and three horizontal gel electrophoresis units for PCR/RT-PCR applications.

BioRad iCycler Unit

Imaging System:

One UVP high-resolution digital imaging system for photographing electrophoresis gels.

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